Custom antibody development  against haptens


Raising antibodies against small molecules

Stop wasting time & money !

 

Maximizing the success rate and the quality of your generated monoclonal antibodies in rats and guinea pigs with multi-species approach.

 

Stay relaxed.

 

Enjoy an unique one-stop-shop solution with dedicated experienced project manager: from animal housing to custom manufacturing up to several grams.

 

 

Targeting lipidspeptidesneoepitopestoxinsepigenetic modifications and polysaccharides,

 

SYnAbs has developed a strong expertise in immunology to break immunotolerance in rat and guinea pig species to generate unique monoclonal antibodies of extreme specificity and higher affinity.


SYnAbs' custom monoclonal antibody generation service is a one-stop shop solution allowing the generation of high quality specific antibodies for in-vitro diagnostic, R&D and therapeutic purposes.

 

Over the years, SynAbs has accumulated extensive know-how in the generation of custom antibodies and the design & selection of antigens, such diverse as:

 

Neo-epitopes to detect specific protein cleavage sites,


Epigenetic modifications including methylated, phosphorylated and acetylated forms,


Conformational epitopes,


Steroids,


Small molecules (drugs...),


Peptides,


Bacteria, Viruses & Toxins (incl. low level detection),


High-homology antigens to distinguish between two closely related isoforms

(site specific mutations, substitution, deletion, insertion...),


Glycans,


Antibodies-related such as anti-idiotypes and anti-isotypes,


Difficult to express proteins (ion channel, GPCR),


Intracellular targets,


Fusion proteins,

 

You'll find here a few cases studies of our tailor-made projects :

Antigen design and production

As a complete service, SYnAbs is able to design, synthesize and conjugate antigens for use as immunogen for your monoclonal antibody development program. Using our in-house synthesis service, SYnAbs will assist you with the selection of antigenic epitope sections and the final peptide will be conjugated to a carrier protein to improve immunogenicity.

Rats & Guinea pigs immunization schedules

SYnAbs offers variable immunization schedules and injection routes to benefit from natural antibody maturity, while breaking immune tolerance for small molecules, peptides and low-immunogenic compounds

 

SYnAbs maximizes the success rate and the quality of generated custom monoclonal antibody by applying parallel multi-species immunization strategy getting access to rat, mouse, and guinea pig immune repertoires. 

 

In case your antigen is expensive, difficult to produce, or is conformation sensitive, SYnAbs will develop a custom DNA immunization approach.

 

Our expertise in the monitoring of antibody-mediated immune response is translated into recommendations prior to fusion step. Our customers are supplied with antiserum sample for screening purposes. In order to boost immune responses, SYnAbs has developed its proprietary special adjuvant.

Fusion with proprietary myeloma cell lines

SYnAbs has generated its own proprietary fusion cell lines in order to offer only stable and high productivity homo-hybridoma. After the fusion, cells are dispensed in 96 well plates (between 10 to 20, depending on initial number of cells). About two weeks later, hybridoma clones begin to appear. When the cells are in confluence, the supernatant will be tested by ELISA immuno-assay.

 

SYnAbs has developed a specific know-how to obtain a high efficiency electrofusion to generate a large number of positive hybridomas. The fusion is performed with all the spleen and poplitea lymph nodes to get an average of positives clones per target. 

Screening against target antigen and cloning

The supernatant of the positive clones will be immediately and regularly sent to your labs to be tested within a week by your own screening method. Your method can also be transferred to our labs at your convenience. 20 positives clones are kept and stored in nitrogen and each clone 3 vials and 10 ml of culture supernatant are prepared. The culture medium is composed by DMEM and FCS supplemented with HAT. After the clones' selection, the HAT is progressively eliminated. All your clones are isotyped and for chosen clones, a subcloning step by limit dilution is realized.

Antibodies animal-free manufacturing and purification

SYnAbs cell culture services offer animal-free tissue culture based protocols in shake-flasks, spinners, CELLines, and FiberCell and provide scale-up activities for large-scale manufacturing up to several grams. We also perform monoclonal antibodies purification and cell storage banking services.

  • Serum-free adaptation,
  • Up-stream & Down-Stream Processing development, scale-up and manufacturing up to several grams,
  • Flexible starting material : hybridoma cells can be developed by SynAbs or provided by the customer,
  • Extensive cell culture expertise 
Up Stream Process mode Quantity of Purified Antibody Timelines
T-Flasks 2 mgrs 2 weeks
Spinner 20 mgrs 4 weeks
CELLine 200 mgrs 8 weeks
FiberCell 500 mgrs to several grams 12 weeks

A large panel of Down-Stream process steps :

o Concentration Diafiltration, TFF

o Affinity chromatography (protein A, G, , specific & proprietary immuno-affinity),

o Clearance solutions for endotoxins removal

 

A large panel of Quality Controls on your batch :

o Aggregates (by SEC-HP C)

o Endotox testing (by LAL)

o Mycoplasma testing

o Bioburden, sterility testing

o Western-Blot

o FACS

o Kinetic features (Kd,Ka) by BLItz & Octet

Development of custom immunoassay

Along the years, SYnAbs has developed a strong expertise in development of custom immunoassay and brings its expertise on the table for the optimization of your developed tests.

Humanization for therapeutic purposes

SYnAbs has a particular know-how to offer CDR grafting performance, humanization technique whereby humanized antibody sequences are generated by selecting CDRs of the parental antibody.

Unique benefits working with SYnAbs

  • Unique success rate approaching 90%, securing a superior return on investment thanks to unique multi-species immunization approach getting access to rat, mouse, and guinea pig immune repertoires.
  • A large track record in the generation of innovative monoclonal antibodies against poor immunogenic compounds and complex antigens.
  • Strong and transparent international reputation. The SynAbs team encourages you to meet our clients they are our best sales persons.
  • As member of mAbExperts, a group of companies with strongly matching expertise, clients can benefit from a one-stop-shop experience from SynAbs, ranging from antigen design to antibody generation, engineering, characterization, production, analytics, assay design & validation.
  • Custom development and manufacturing capabilities coupled with off-the-shelf singular antibodies references: combined solutions that grow with your business
  • Quality environment covered by ISO : certification, SynAbs complies with high-quality standards.