Aflatoxins are a worldwide health problem as many as 5 billion persons may be exposed. Children are particularly affected by aflatoxin exposure, which leads to stunted growth, delayed development, liver damage and liver cancer. Adults have a higher tolerance but are also at risk. Aflatoxins have a negative economic impact on agriculture through reduced marketing options for crops and adverse health effects on livestock. Losses due to aflatoxins annually cost $ 900 million in Indonesia, the Philippines and Thailand alone.
Rat mAb displays a better affinity than the mouse one. Actually, 50% of ELISA signal is lost at a 60 ng/ml dilution for MA-afla, whereas for the LOU-afla mAb the signal loss occurs at 15 ng/ml.
Competitive assay shows that rat mAb has a better sensitivity than the mouse mAb for free aflatoxin.
Aflatoxin B1 conjugated with BSA as coated at 5 μg/ml on ELISA plate. Decreasing concentration of MA-afla and LOU-afla were incubated. Revelation has been performed with mouse anti-rat kappa light chain and rat anti-mouse kappa light chain HRP conjugated.
500 μg/ml of MA-afla and 125 ng/ml of LOU-afla were incubated on the plate where 5 μg/ml of BSA-aflatoxin were coated. A range of concentration (1000 - 2 ng/ml) of free aflatoxin B1 was used to shift the balance between BSA-aflatoxin/mAb. Revelation has been performed with a mouse anti-rat kappa light chain and rat anti-mouse kappa light chain HRP conjugated.
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