LO-MM-3, developed by SynAbs, is a rat anti-mouse IgM monoclonal antibody designed specifically for R&D use only (RUO). Validated in peer-reviewed research, including studies of B-cell proliferation and immunoglobulin production, LO-MM-3 is a key reagent in in vitro diagnostics, ELISA quantification, and immunoassays targeting murine IgM isotypes.
Why LO-MM-3 Is a Reference Antibody for IgM Detection in Mouse Models
The immune response begins with IgM — the first antibody secreted by B cells upon activation. Detecting and quantifying murine IgM provides key insights into:
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B-cell development and activation
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Antibody class switching
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Early immune response in allergy, infection, and vaccination models
LO-MM-3 offers high specificity, minimal cross-reactivity, and excellent performance in coating ELISA plates for murine IgM detection.
LO-MM-3 in Research: Real-World Applications in Immunology
LO-MM-3 has been successfully used in numerous peer-reviewed studies. One notable example is:
Savignac et al., 2010 — "Increased B Cell Proliferation and Reduced Ig Production in DREAM Transgenic Mice"
Objective
To study how expression of a dominant active mutant of DREAM, a transcriptional repressor, affects B-cell function.
How LO-MM-3 Was Used
Researchers used LO-MM-3 as a coating antibody in ELISA to measure serum IgM levels in both wild-type and transgenic mice. This allowed precise quantification of IgM production in response to LPS and IL-4 stimulation
Findings Supported by LO-MM-3
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DREAM-transgenic B cells produced significantly lower IgM, IgG1, IgG2a, and IgE compared to controls.
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LO-MM-3 enabled the detection of IgM secretion defects not explained by transcriptional failure, but linked to reduced translation.
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This was essential in establishing that DREAM affects protein synthesis machinery in B cells.
Key Result
"Using ELISA plates coated with rat anti-mouse IgM (LO-MM-3), we confirmed that transgenic B cells have a significantly reduced capacity to secrete IgM in vitro, despite normal differentiation markers."
Technical Specifications
| PROPERTY | Description |
| Clone | LO-MM-3 |
| Isotype | Rat IgG2a |
| Host | Rat-LOU |
| Specificity | Mouse IgM |
| Application | ELISA, Flow Cytometry, Immunoassay (RUO) |
| Concentration | ~1 mg/mL (standard format) |
| Format | Purified, carrier-free, azide-free optional |
Optimized for Research Use Only (RUO)
LO-MM-3 is strictly intended for in vitro research applications:
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Immunoassay Development
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Murine Model Evaluation
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Basic Immunology R&D
- Diagnostic kit
It is not intended for use in humans.
Frequently Used With
| MONOCLONAL ANTIBODY | Target isotype |
| LO-ME-3 | Mouse IgE |
| LO-MG1-13 | Mouse IgG1 |
| LO-MG2a-9 | Mouse IgG2a |
| LO-MK-1 | Mouse kappa light chain |
Trusted by Researchers Worldwide
LO-MM-3 is used by immunologists and molecular biologists across leading research institutes for:
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Antibody class switching studies
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Flow cytometry profiling
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ELISA-based quantification
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Evaluation of immune dysregulation (e.g., in genetically modified mice)
