MARK is a high-specificity mouse monoclonal antibody developed by SynAbs to target the κ light chain of rat immunoglobulins (Igκ). Designed for Research Use Only (RUO), MARK serves critical functions in:

• ✅ Immunoassays: as a secondary antibody in ELISA, Western blot, and flow cytometry

• ✅ Antibody purification: in immunoaffinity chromatography using Sepharose coupling

• ✅ Lateral flow tests (LFIAs): as a control line capture antibody for rat monoclonal detection

This triple utility makes MARK a cornerstone reagent for immunologists, CROs, and diagnostics developers.

When using rat monoclonal antibodies to detect antigens in mouse models, researchers rely on MARK to detect those antibodies with high specificity.

🔬 Case: Differentiating Rat B Cells
📄 Differentiation of membrane IgE+ rat B cells into IgE-secreting cells

Researchers used rat IgE-specific monoclonals in ELISA; detection required an anti-Igκ secondary like MARK for signal amplification.

🔬 Case: CD23 Expression in Aged Rats
📄 CD23 expression in aged rats

Flow cytometry panels used rat primaries; MARK enabled precise detection of surface-bound rat Ig via κ chain recognition.

 

In rapid tests, when the primary antibody is a rat monoclonal, MARK is often used at the control line to ensure proper flow and capture of unbound antibody.

🧪 Example Application:

• A rat monoclonal antibody is conjugated to colloidal gold or latex and added to the test pad.

• MARK is immobilized at the control line.

• If the system works properly, the rat antibody (free or antigen-bound) binds MARK, producing a visible control line — validating the assay's performance.

This ensures:

• Test validity (internal control)

• Clean, reproducible capture of rat monoclonals

• Compatibility with standard conjugation chemistries

MARK is widely used by diagnostics manufacturers building RUO and prototype LFIAs involving rat IgG1 or IgG2a monoclonals.

MARK can be covalently coupled to Sepharose 4B or similar matrices for the affinity purification of rat immunoglobulins from:

• Hybridoma culture supernatants

• Rat serum or ascitic fluid

📄 Publications that reference or enable this usage:

• Rat monoclonal antibodies. I & II – purification from supernatants

• V. A simple automated technique of antigen purification – immunoaffinity strategy

• Purification of rat monoclonal antibodies – rapid isolation using κ light chain capture

Researchers using these methods were able to purify highly specific monoclonals for use in transplant models, allergy assays, and antigen-specific labeling.

MARK or similar κ-specific antibodies are cited in key studies:

• Fc epsilon receptors on rat B cells – signal detection via κ

• B cell IgE switching – secondary detection in ELISA

• Immunoaffinity purification – κ coupling for column-based Ig recovery

• Autoimmune and transplant models – purified rat IgG via κ-capture for in vivo use

Each example confirms MARK's role as a trusted research reagent in both functional studies and technical workflows.

MARK is available in multiple formats:

• 📦 1 mg vials, custom Bulk upon request

• 🎯 Conjugated versions: HRP, Biotin, Alexa Fluor®

• 🧼 Carrier-free for covalent immobilization

MARK is a powerful and versatile monoclonal antibody designed for:

• Detection of rat primary antibodies

• Use in LFIAs as a control line antibody

• Affinity purification of rat Ig via kappa recognition

With proven specificity, flexible formats, and compatibility across platforms, MARK enables precision across immunoassays and antibody workflows.

🔬 Make MARK your go-to anti-rat Igκ tool for R&D that flows, captures, and delivers.