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LO-HG4 Anti-human IgG4 monoclonal antibody

“LO-HG4”Anti-human IgG4 monoclonal antibody New SYngular product from SYnAbs

SYnAbs (www.synabs.be) is proud to announce the launch of new high added value products: two monoclonals from rat-LOU species highly specific to human IgG4 subclass, without any cross reaction with other human isotypes.

 

Immunoglobulin G (IgG) is one of the most abundant proteins in human serum, accounting for about 15% of all plasma protein content. Humans express four types of immunoglobulin G, known as IgG1, IgG2, IgG3, and IgG4.

 

IgG4 present conformational differences with IgG1 Cc2 domain loops. While the overall domain structure is conserved, the conformation of BC and FG loops is different, and in IgG4, the FG loop folds away from the Cc2 domain. As the differences are very slight, it’s very difficult to create anti-IgG4 antibody that doesn’t cross-react with the other IgG classes.

 

We conjugated our anti-IgG4 to HRP probe since we wanted to be sure to have similar outcomes with unconjugated and conjugated antibodies.

  

Coating: Human IgG1, IgG2, IgG3 from Sigma, IgG4 from Abcam. 5 μg/ml.

LO-HG4-clone#1. 1mg/ml

Clone#1. dilution/coating

IgG1

IgG2

IgG3

IgG4

2000   

0,090

0,100

0,080

2,590

4000   

0,070

0,080

0,080

2,270

8000   

0,070

0,070

0,060

1,900

16000   

0,060

0,060

0,060

1,310

32000   

0,060

0,060

0,060

0,850

64000   

0,060

0,060

0,060

0,580

128000   

0,060

0,060

0,070

0,360

256000   

0,060

0,060

0,060

0,230

512000   

0,060

0,060

0,060

0,150

0   

0,060

0,060

0,060

0,090

       
         

LO-HG4-clone#5. 1 mg/ml

Clone#5. dilution/coating

IgG1

IgG2

IgG3

IgG4

2000   

0,100

0,150

0,110

2,730

4000   

0,080

0,110

0,090

2,400

8000   

0,070

0,090

0,070

1,900

16000   

0,070

0,070

0,070

1,320

32000   

0,060

0,070

0,060

0,830

64000   

0,060

0,060

0,060

0,500

128000   

0,060

0,060

0,060

0,300

256000   

0,060

0,060

0,060

0,200

512000   

0,060

0,060

0,060

0,150

0   

0,060

0,060

0,060

0,060

 
   

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https://www.synabs.be/about-synabs/contact-synabs/