Guinea pig Monoclonal Antibodies

Mouse monoclonal antibodies are standard in research and diagnostic. Among all advantages of mouse monoclonal antibodies there is a lack of affinity, sensitivity and specificity especially for small epitopes and antigens that are non-immunogenic to rodents. This suggests that a huge number of diseases are under-diagnosed because of non-availability of high-quality antibodies.

 

Generating monoclonal antibodies from the guinea pig is a breakthrough. Moreover this cutting edge technology could rapidly take the market share of the disappointing rabbit monoclonals, featuring sometimes good affinity but often very poor productivity and being unstable.

 

SynAbs has therefore opted to develop its own Syn2.2 myeloma cell line to manufacture guinea pig monoclonal antibodies and offer more efficient investigation tools for researchers. 


Benefits of guinea pig mAbs

Guinea pig mAbs are the best of both worlds, offering stability together with a non-murine immune repertoire (outbreed).

 

Guinea pig monoclonal antibodies show 10-100 times higher affinities and impress by improved specificity compared to mouse monoclonal antibodies. That makes guinea pig mAbs extremely important in all fields of clinical and non-clinical diagnostic

 

The Guinea pig model should be preferentially used when the target is :

  • highly conserved between mouse/rat/rabbit/human 
  • a glycemic hormone, proven to be different in the guinea pig 
  • an aflavirus antigen, the guinea pig being used as a preclinical model

Achievement 1/2

After a year of research, SynAbs has managed to develop a fusion cell line, called Syn2.2, so that guinea pig B lymphocytes can be fused efficiently to obtain hybridomas that produce monoclonal antibodies in a stable way. Around 107 splenocytes can be isolated in a guinea pig spleen, a comparable number to rats but around twice that for mice.

The Syn2.2 fusion rate with guinea pig splenocytes is comparable to that obtained with SP2/0 for mice or IR983 for rats, i.e. approximately 12 hybridomas per million fused splenocytes, which means that more than 1000 hybridomas can be obtained from a guinea pig spleen. After analysis, we observed that nearly 50% of the hybridomas produced an antibody, with production rates comparable to those for mice and rats.  

Achievement 2/2

Here are the humoral responses of a guinea pig and a rat immunized in the same way with a steroid coupled to KLH and determined by titration on the same steroid coupled to ovalbumin.    

It has been observed on many occasions that the humoral response to a particular antigen was on average at least two times higher in guinea pigs than in rats or mice.